Integration host factor facilitates repression of the put operon in Salmonella typhimurium.
Gene
; 118(1): 13-9, 1992 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-1511875
ABSTRACT
Transcriptional regulation of the put operon is mediated by a unique mechanism involving autogenous regulation by the PutA protein, a membrane-associated dehydrogenase. The 420-bp put control region contains the putP and putA promoters, multiple operator sites, multiple catabolite repression protein binding sites, and several potential integration host factor (IHF)-binding sites (ihf). In this study, we show that IHF facilitates repression of the put operon in vivo, and IHF binds specifically to two ihf sites in the put control region in vitro. DNA gyrase mutants that alter the degree of chromosomal supercoiling do not affect put regulation, indicating that the effect of IHF on put expression is in this case independent of supercoiling.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Óperon
/
Salmonella typhimurium
/
Proteínas de Bactérias
/
Prolina
/
Sequências Reguladoras de Ácido Nucleico
/
Repressão Enzimática
Idioma:
En
Revista:
Gene
Ano de publicação:
1992
Tipo de documento:
Article