Inkjet printing of viable mammalian cells.
Biomaterials
; 26(1): 93-9, 2005 Jan.
Article
em En
| MEDLINE
| ID: mdl-15193884
ABSTRACT
The purpose of this study was to explore the use of a commercial thermal printer to deposit Chinese Hamster Ovary (CHO) and embryonic motoneuron cells into pre-defined patterns. These experiments were undertaken to verify the biocompatibility of thermal inkjet printing of mammalian cells and the ability to assemble them into viable constructs. Using a modified Hewlett Packard (HP) 550C computer printer and an HP 51626a ink cartridge, CHO cells and rat embryonic motoneurons were suspended separately in a concentrated phosphate buffered saline solution (3 x). The cells were subsequently printed as a kind of "ink" onto several "bio-papers" made from soy agar and collagen gel. The appearance of the CHO cells and motoneurons on the bio-papers indicated an healthy cell morphology. Furthermore, the analyses of the CHO cell viability showed that less than 8% of the cells were lysed during printing. These data indicate that mammalian cells can be effectively delivered by a modified thermal inkjet printer onto biological substrates and that they retain their ability to function. The computer-aided inkjet printing of viable mammalian cells holds potential for creating living tissue analogs, and may eventually lead to the construction of engineered human organs.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sobrevivência Celular
/
Periféricos de Computador
/
Técnicas de Cultura de Células
/
Engenharia Tecidual
/
Células do Corno Anterior
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
Biomaterials
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos