Sequential Peptide Affinity (SPA) system for the identification of mammalian and bacterial protein complexes.
J Proteome Res
; 3(3): 463-8, 2004.
Article
em En
| MEDLINE
| ID: mdl-15253427
ABSTRACT
A vector system is described that combines reliable, very low level, regulated protein expression in human cells with two affinity purification tags (Sequential Peptide Affinity, or SPA, system). By avoiding overproduction of the target protein, this system allows for the efficient purification of natural protein complexes and their identification by mass spectrometry. We also present an adaptation of the SPA system for the efficient purification and identification of protein complexes in E. coli and, potentially, other bacteria.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Proteínas Recombinantes
/
Fatores de Transcrição TFII
/
Complexos Multiproteicos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Proteome Res
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2004
Tipo de documento:
Article