Homocysteine increases methionine synthase mRNA level in Caco-2 cells.
Cell Physiol Biochem
; 14(4-6): 407-14, 2004.
Article
em En
| MEDLINE
| ID: mdl-15319544
ABSTRACT
BACKGROUND:
Methionine synthase (MTR) synthesizes methionine from homocysteine, using cobalamin as a cofactor and 5-methyltetrahydrofolate as a cosubstrate.AIM:
To determine the influence of homocystine (Hcy, dimer of homocysteine) in the presence of either cobalamin or methionine on the transcription and the activity of methionine synthase in Caco-2, a human adenocarcinoma cell line.METHODS:
Methionine synthase activity and quantification of its mRNA by real-time RT-PCR were determined in cells cultivated under four differents conditions Hcy with cobalamin (Hcy+ Cbl+), Hcy with methionine (Hcy+Met+), methionine with Cbl (Met+ Cbl+) and methionine only (Met+).RESULTS:
Activity (nmol/h/mg protein) was maximal in cells cultivated in Hcy+Cbl+ (2.45 +/- 0.35), compared to cells cultivated in Hcy+Met+ (0.18 +/- 0.01, p<0.001), in Met+ Cbl+ (1.60 +/- 0.06, p<0.05), and in Met+ (0.40 +/- 0.05, p<0.001), suggesting an adaptation of the cells to requirement in synthesized methionine. The mRNA level of MTR in Hcy+ Cbl+ and Hcy+Met+ (2.82 +/-0.49 and 3.33 +/- 0.48 AU, respectively ) was about 2.5 / 3.0-fold higher than that in Met+ Cbl+ and in Met+ (1.00 +/-0.13 and 1.20 +/-0.20 AU, respectively, p<0.001).CONCLUSION:
Methionine synthase expression of Caco-2 cell is under a transcriptional control influenced by Hcy.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
5-Metiltetra-Hidrofolato-Homocisteína S-Metiltransferase
/
Adenocarcinoma
/
Regulação Neoplásica da Expressão Gênica
/
Regulação para Cima
/
Homocisteína
Limite:
Humans
Idioma:
En
Revista:
Cell Physiol Biochem
Assunto da revista:
BIOQUIMICA
/
FARMACOLOGIA
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
França