Dimethylthiarum disulfide: new sulfur transfer reagent in phosphorothioates oligonucleotide synthesis.
Methods Mol Biol
; 288: 51-64, 2005.
Article
em En
| MEDLINE
| ID: mdl-15333897
ABSTRACT
Dimethylthiarum disulfide (DTD) has been developed as a new and efficient sulfur-transfer reagent for automated synthesis of phosphorothioate oligonucleotides using phosphoramidite chemistry. The traditional four-step automated oligonucleotide synthesis has been compressed to three-step protocol using DTD. This improvement allowed an overall 20% reduction in the solvent consumption and reduced the total synthesis time by 25%. The large-scale application of DTD has been successfully demonstrated by synthesis of therapeutically useful 20-mer phosphorothioate antisense oligonucleotides with excellent yield and purity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Enxofre
/
Tiocarbamatos
/
Tionucleotídeos
/
Indicadores e Reagentes
Tipo de estudo:
Guideline
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos