Identification and subcellular distribution of endogenous Ins(1,4,5)P(3) 3-kinase B in mouse tissues.
Biochem Biophys Res Commun
; 323(3): 920-5, 2004 Oct 22.
Article
em En
| MEDLINE
| ID: mdl-15381088
ABSTRACT
Inositol 1,4,5-trisphosphate 3-kinase (IP(3)-3K) catalyses the phosphorylation of inositol 1,4,5-trisphosphate to inositol 1,3,4,5-tetrakisphosphate. cDNAs encoding three mammalian isoforms have been reported and referred to as IP(3)-3KA, IP(3)-3KB, and IP(3)-3KC. IP(3)-3KB is particularly sensitive to proteolysis at the N-terminus, a mechanism known to generate active fragments of lower molecular mass. Endogenous IP(3)-3KB has therefore not been formally identified in tissues. We have probed a series of murine tissues with an antibody directed against the C-terminus of IP(3)-3KB and used IP(3)-3KB deficient mouse tissues as negative controls. IP(3)-3KB was shown to be particularly well expressed in brain, lung, and thymus with molecular masses of 110-120kDa. The identification of the native IP(3)-3KB by Western blotting for the first time will facilitate further studies of regulation of its activity by specific proteases and/or phosphorylation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Frações Subcelulares
/
Timo
/
Encéfalo
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Fosfotransferases (Aceptor do Grupo Álcool)
/
Pulmão
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Bélgica