Linkage disequilibrium between MSX1 and non-syndromic cleft lip/palate in the Chilean population.
J Dent Res
; 83(10): 782-5, 2004 Oct.
Article
em En
| MEDLINE
| ID: mdl-15381719
ABSTRACT
Non-syndromic cleft lip/palate (NSCLP) is a complex genetic trait. Linkage and association studies have suggested that a clefting locus could be located on chromosome 4p. Sixty Chilean families were recruited for this study; from these, we used unrelated trios to evaluate the possible linkage disequilibrium between MSX1 and NSCLP. An intragenic marker, MSX1-CA, and an extragenic marker, D4S432 at a distance of 0.8 cM from MSX1, were analyzed by means of polymerase chain-reaction with fluorescent-labeled forward primers, followed by electrophoresis on a laser-fluorescent sequencer. We carried out a transmission/disequilibrium test (TDT) for multiple alleles to evaluate the presence of linkage disequilibrium. Results showed a preferential transmission of the 169-bp allele of MSX1 (p = 0.03). Although there was no preferential transmission for the D4S432 marker, the overall extended TDT (ETDT) showed a significant result (p = 0.01). The authors' findings support the hypothesis of the contribution of MSX1 in the etiology of NSCLP in the Chilean population.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Cromossomos Humanos Par 4
/
Fenda Labial
/
Fissura Palatina
/
Proteínas de Homeodomínio
Tipo de estudo:
Health_economic_evaluation
Limite:
Adult
/
Child
/
Child, preschool
/
Humans
País/Região como assunto:
America do sul
/
Chile
Idioma:
En
Revista:
J Dent Res
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Chile