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rDNA-RFLP identification of Candida species in immunocompromised and seriously diseased patients.
Pinto, Patrícia M; Resende, Maria A; Koga-Ito, Cristiane Y; Ferreira, José A G; Tendler, Miriam.
Afiliação
  • Pinto PM; Departament de Microbiologia--Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Can J Microbiol ; 50(7): 514-20, 2004 Jul.
Article em En | MEDLINE | ID: mdl-15381977
PCR was used to amplify a targeted region of the ribosomal DNA of 76 Candida spp. isolates from immunocompromised and seriously diseased patients. Thirty-seven strains isolated from different anatomical sites of 11 patients infected with HIV (Vitória, ES, Brazil), 26 isolates from patients under treatment at Odilon Behrens Hospital and 13 isolates from skin and urine samples from São Marcos Clinical Analysis Laboratory (Belo Horizonte, Brazil) were scored. Fragments of rDNA were amplified using primer pairs ITS1-ITS4, for the amplification of ITS1 and ITS2 regions, including the gene for the 5.8 s subunit. Amplification resulted in fragments ranging in size from 350 to 950 bp. Amplicons were digested with eight restriction enzymes. A pattern of species-specificity among the different medically important Candida species could be identified following restriction digestion of the PCR products. Candida albicans was the species most frequently observed, except for the group of newborns under treatment at the Odilon Behrens Hospital and for the isolates from the clinical analysis laboratory. C. parapsilosis was the species most frequently observed in these two groups.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polimorfismo de Fragmento de Restrição / Candida / Candidíase / DNA Ribossômico / Hospedeiro Imunocomprometido Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Can J Microbiol Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Brasil
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polimorfismo de Fragmento de Restrição / Candida / Candidíase / DNA Ribossômico / Hospedeiro Imunocomprometido Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Can J Microbiol Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Brasil