The patch-clamp and planar lipid bilayer techniques: powerful and versatile tools to investigate the CFTR Cl- channel.
J Cyst Fibros
; 3 Suppl 2: 101-8, 2004 Aug.
Article
em En
| MEDLINE
| ID: mdl-15463939
ABSTRACT
Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism of action, interaction with other proteins and physiological role. Using the PLB technique, the structure and function of CFTR can be investigated free from the influence of other proteins. Here we discuss how these techniques are employed to investigate the CFTR Cl- channel with special emphasis on its permeation, conduction and gating properties.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas de Patch-Clamp
/
Regulador de Condutância Transmembrana em Fibrose Cística
/
Bicamadas Lipídicas
Limite:
Humans
Idioma:
En
Revista:
J Cyst Fibros
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Reino Unido