Comparison of a monoclonal antibody fluorescent polarization immunoassay with monoclonal antibody radioimmunoassay for cyclosporin determination in whole blood.
Ther Drug Monit
; 14(1): 78-80, 1992 Feb.
Article
em En
| MEDLINE
| ID: mdl-1546394
ABSTRACT
We have compared the whole blood concentrations of parent cyclosporin A (CsA) using monoclonal fluorescence polarization immunoassay (FPIA) and radioimmunoassay (RIA) as well as polyclonal FPIA in kidney, heart, and bone marrow transplant patients (n = 89). A good correlation was found between monoclonal FPIA and monoclonal RIA (r = 0.96) and a slightly worse one between polyclonal and monoclonal FPIA (r = 0.90). The interassay coefficient of variation was satisfactory for all the methods-less than 5% for monoclonal FPIA. The monoclonal FPIA assay with Abbott TDx appears to provide rapid, precise, and accurate measurement of parent CsA. It is therefore useful for therapeutic monitoring of CsA in whole blood in kidney, heart, and bone marrow transplant patients.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ciclosporina
Limite:
Humans
Idioma:
En
Revista:
Ther Drug Monit
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Espanha