A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumors.
Cancer Res
; 64(19): 7022-9, 2004 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-15466195
ABSTRACT
Invasion of tumor cells into the surrounding connective tissue and blood vessels is a key step in the metastatic spread of breast tumors. Although the presence of macrophages in primary tumors is associated with increased metastatic potential, the mechanistic basis for this observation is unknown. Using a chemotaxis-based in vivo invasion assay and multiphoton-based intravital imaging, we show that the interaction between macrophages and tumor cells facilitates the migration of carcinoma cells in the primary tumor. Gradients of either epidermal growth factor (EGF) or colony-stimulating factor 1 (CSF-1) stimulate collection into microneedles of tumor cells and macrophages even though tumor cells express only EGF receptor and macrophages express only CSF-1 receptor. Intravital imaging shows that macrophages and tumor cells migrate toward microneedles containing either EGF or CSF-1. Inhibition of either CSF-1- or EGF-stimulated signaling reduces the migration of both cell types. This work provides the first direct evidence for a synergistic interaction between macrophages and tumor cells during cell migration in vivo and indicates a mechanism for how macrophages may contribute to metastasis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quimiotaxia
/
Fator Estimulador de Colônias de Macrófagos
/
Fator de Crescimento Epidérmico
/
Macrófagos
/
Neoplasias Mamárias Experimentais
Limite:
Animals
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Estados Unidos