Development of an accelerated low-pH reversed-phase liquid chromatography column stability test.
J Chromatogr A
; 1060(1-2): 153-63, 2004 Dec 10.
Article
em En
| MEDLINE
| ID: mdl-15628158
ABSTRACT
The important experimental design criteria for an accelerated low-pH RPLC column stability test are discussed. The influence of method variables on the amount and rate of retention-loss and the final optimized parameters for the accelerated low-pH RPLC stability test are presented. The retention-loss curves for selected C8 and C18 stationary phases are compared. These studies indicate that ligand chain length, functionality and bonding density play an important role in determining the low-pH stability of a stationary phase. Additionally, elemental analysis data are used to infer the mechanism responsible for the observed retention-loss under low-pH conditions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Concentração de Íons de Hidrogênio
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Estados Unidos