[Polymorphism analysis of the goat growth hormone gene in the 5' regulatory sequence].
Yi Chuan
; 26(6): 831-5, 2004 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-15640112
ABSTRACT
The polymorphism of goat growth hormone gene in 5'-region in 274 goats including Lubei white goat, introducted Boer goat, pure bred Boer goat , hybrid generation 1 of LuBei white goat and Boer goat , backcross of hybrid generation 1 and Boer goat were detected by PCR-SSCP with two different pairs of primers. The result showed that in 26-239bp most of the Boer goats and hybrid generation presented as AA genotype,while most LuBei white goats presented as BB genotype. In 225-429bp all breeds presented as more CC genotype. The amplified fragments of AA,BB and CC,DD of the two fragments were cloned and sequenced . The result showed that in 26-239bp AA genotype had one substitution mutation at 60 site (C-->T), and base C losing at 211 site and DD genotype had three substitution mutations at 264 site (T-->C), 292 site (T-->A) and 372 site (C-->T) in 225-429bp. The results above-mentioned first confirmed that there were polymorphisms in 5' region of GH gene.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polimorfismo Genético
/
Cabras
/
Hormônio do Crescimento
/
Região 5'-Flanqueadora
Limite:
Animals
Idioma:
Zh
Revista:
Yi Chuan
Assunto da revista:
GENETICA
Ano de publicação:
2004
Tipo de documento:
Article