Effect of DNA structure on the formation of collagen-DNA complex.
Int J Biol Macromol
; 35(1-2): 39-46, 2005 Mar.
Article
em En
| MEDLINE
| ID: mdl-15769514
ABSTRACT
Using various types of DNAs prepared from plasmid DNA, complete double-stranded DNA (ds.DNA) with linear and cyclic forms and double-stranded DNA coexisting with single-stranded DNA (ss.DNA), the structure and fibrillogenesis of the collagen-DNA complex were investigated by means of turbidity, transmission electron microscopy, and confocal laser-scanning microscopy. The rate of fibrillogenesis of the collagen-DNA complex significantly depends on the DNA structure. The structure of the fibrils formed in the complexes showed a marked difference between the ds.DNA and ss.DNA complexes with collagen. Spatial distribution of the DNA and collagen in the complexes suggests that the characteristic collagen-DNA interaction depends on the DNA forms.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Materiais Biocompatíveis
/
DNA
/
DNA de Cadeia Simples
/
Colágeno
/
Conformação de Ácido Nucleico
Idioma:
En
Revista:
Int J Biol Macromol
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Japão