Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans.
Nature
; 434(7032): 462-9, 2005 Mar 24.
Article
em En
| MEDLINE
| ID: mdl-15791247
ABSTRACT
A key challenge of functional genomics today is to generate well-annotated data sets that can be interpreted across different platforms and technologies. Large-scale functional genomics data often fail to connect to standard experimental approaches of gene characterization in individual laboratories. Furthermore, a lack of universal annotation standards for phenotypic data sets makes it difficult to compare different screening approaches. Here we address this problem in a screen designed to identify all genes required for the first two rounds of cell division in the Caenorhabditis elegans embryo. We used RNA-mediated interference to target 98% of all genes predicted in the C. elegans genome in combination with differential interference contrast time-lapse microscopy. Through systematic annotation of the resulting movies, we developed a phenotypic profiling system, which shows high correlation with cellular processes and biochemical pathways, thus enabling us to predict new functions for previously uncharacterized genes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genoma
/
Caenorhabditis elegans
/
Proteínas de Caenorhabditis elegans
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Interferência de RNA
/
Desenvolvimento Embrionário
Limite:
Animals
Idioma:
En
Revista:
Nature
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Alemanha