Quantification of imidacloprid toxicity to avocado thrips, Scirtothrips perseae Nakahara (Thysanoptera: Thripidae), using a combined bioassay and ELISA approach.
Pest Manag Sci
; 61(8): 754-8, 2005 Aug.
Article
em En
| MEDLINE
| ID: mdl-15834841
ABSTRACT
A competitive ELISA (enzyme-linked immunosorbent assay) technique was evaluated for quantifying titres of imidacloprid in homogenates of leaf discs sampled from avocado plants treated with systemic applications of imidacloprid 240 g litre(-1) SC (Admire). Matrix effects were evident with undiluted leaf tissue homogenates, but these were effectively eliminated by dilution of homogenates in water. In a field trial conducted in a commercial nursery, there was an excellent correlation between imidacloprid residues within leaves and avocado thrips (Scirtothrips perseae Nakahara) mortality. However, with subsequent sampling over a 2-month period, the relationship between mortality and apparent imidacloprid concentration became less robust, suggesting that the material was being degraded within the plant to non-toxic metabolites. Nevertheless, assessments of thrips mortality on leaves that had been recently treated with imidacloprid established a lower threshold of activity for imidacloprid residues of 6 ng cm(-2) leaf. Limitations on the use of ELISA to quantify the impact of systemic insecticides on pest populations are discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imidazóis
/
Inseticidas
/
Insetos
Limite:
Animals
Idioma:
En
Revista:
Pest Manag Sci
Assunto da revista:
TOXICOLOGIA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos