Combinatorial expression of phenotypes of different cell lineages in the rat and mouse pituitary.
Ann N Y Acad Sci
; 1040: 84-8, 2005 Apr.
Article
em En
| MEDLINE
| ID: mdl-15891010
ABSTRACT
As studied by single cell RT-PCR of pituitary hormones, we demonstrated that the pituitaries of rats and mice contain a subpopulation of cells that express two or more hormone phenotypes typically belonging to lineages that are branched separately early during embryonic development, such as glycoprotein hormone alpha-subunit (alphaGSU) mRNA + PRL mRNA, alphaGSU mRNA + POMC mRNA, and POMC mRNA + GH or PRL mRNA. GnRH in vitro selectively expands the population of cells coexpressing alphaGSU mRNA + PRL mRNA, and CRH selectively increases the proportion of cells coexpressing alphaGSU mRNA + POMC mRNA. Colocalization of alphaGSU + PRL or alphaGSU + POMC could not be detected by double immunofluorescence. This lineage promiscuity was also observed in the pituitary in vivo.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fenótipo
/
Hipófise
/
Hormônios Hipofisários
/
Linhagem da Célula
/
Técnicas de Química Combinatória
Limite:
Animals
Idioma:
En
Revista:
Ann N Y Acad Sci
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Bélgica