In vitro effects of keyhole limpet hemocyanin in breast and pancreatic cancer in regards to cell growth, cytokine production, and apoptosis.
Am J Surg
; 189(6): 680-4, 2005 Jun.
Article
em En
| MEDLINE
| ID: mdl-15910720
ABSTRACT
BACKGROUND:
We have previously shown the inhibitory effects of keyhole limpet hemocyanin (KLH) against breast and pancreatic cancer in vitro. We hypothesize that its actions in breast and pancreas cancer cells are via apoptotic or cytokine pathways.METHODS:
Two breast cancer cell lines, ZR75-1 and MCF-7, and one pancreas cancer cell line, PANC-1, were treated with KLH at 500 mug, 250 mug, and 250 ng/mL. Cell viability, cytokine production, and apoptosis were measured.RESULTS:
Significant growth inhibition was observed in all cell lines at all KLH concentrations tested. Significant changes in cytokine production were observed in all cell lines. An increase in early and late apoptotic activity was observed in the MCF-7, whereas a reduction in late apoptotic activity was observed in the ZR75-1 cells.CONCLUSIONS:
KLH directly inhibits the growth of human breast and pancreas cancer in vitro by apoptotic and nonapoptotic mechanisms.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Pancreáticas
/
Neoplasias da Mama
/
Hemocianinas
/
Adjuvantes Imunológicos
Limite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Am J Surg
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos