[Effect of IL-24 gene expression on the growth of glioma cells in vitro and in vivo].

ABSTRACT

OBJECTIVE: To investigate the effect of IL-24 expression on the growth of glioma cells. METHODS: The IL-24 gene was transfected into rat glioma C6 cells with a retroviral vector. The expression of IL-24 in C6/IL-24 glioma cells was confirmed by RT-PCR. MTT assay and flow cytometry were used to study tumor cell proliferation in vitro. Tumorigenicity in vivo was studied in inbred SD male rats by the growth of intracerebrally inoculated tumor. RESULTS: It was confirmed by RT-PCR that the exogenous IL-24 gene expressed in C6/IL-24 cell. The C6/IL-24 cell proliferation in vitro and tumorigenicity in vivo were both inhibited compared with its parental C6 cell. CONCLUSION: IL-24 expression in glioma cells somehow inhibits their growth in vitro and in vivo.