ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation.
Mol Cell Biol
; 25(15): 6639-48, 2005 Aug.
Article
em En
| MEDLINE
| ID: mdl-16024799
ABSTRACT
ING2 is a candidate tumor suppressor gene that can activate p53 by enhancing its acetylation. Here, we demonstrate that ING2 is also involved in p53-mediated replicative senescence. ING2 protein expression increased in late-passage human primary cells, and it colocalizes with serine 15-phosphorylated p53. ING2 and p53 also complexed with the histone acetyltransferase p300. ING2 enhanced the interaction between p53 and p300 and acted as a cofactor for p300-mediated p53 acetylation. The level of ING2 expression directly modulated the onset of replicative senescence. While overexpression of ING2 induced senescence in young fibroblasts in a p53-dependent manner, expression of ING2 small interfering RNA delayed the onset of senescence. Hence, ING2 can act as a cofactor of p300 for p53 acetylation and thereby plays a positive regulatory role during p53-mediated replicative senescence.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Nucleares
/
Transativadores
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Proteína Supressora de Tumor p53
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Senescência Celular
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Receptores Citoplasmáticos e Nucleares
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Proteínas de Homeodomínio
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Proteínas Supressoras de Tumor
Limite:
Humans
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos