In vitro echogenicity characterization of poly[lactide-coglycolide] (plga) microparticles and preliminary in vivo ultrasound enhancement study for ultrasound contrast agent application.
Invest Radiol
; 40(8): 536-44, 2005 Aug.
Article
em En
| MEDLINE
| ID: mdl-16024992
ABSTRACT
OBJECTIVES:
This work includes (1) the characterization of a reproducible poly[lactide-coglycolide] (PLGA) microparticle preparation with an optimial mean diameter and size distribution and (2) the preliminary in vivo ultrasonographic investigation of PLGA microparticles.METHODS:
A first series of PLGA microparticle preparations (1 to 15 mum) was acoustically characterized on a hydrodynamic device to select the most appropriate for ultrasound contrast agent application. Preparations of 3-microm microparticles were selected, characterized at different doses, and then injected into 20 melanoma grafted mice for contrast-enhanced power Doppler ultrasonography evaluation.RESULTS:
The 3-microm microparticles (3.26-microm mean diameter with 0.41-microm standard deviation) led to in vitro enhancement of 18.3 dB at 0.62 mg/mL. In vivo experiments showed 47% enhancement of intratumoral vascularization detection after PLGA injection, significantly correlated (P < 0.0001) with preinjection intravascularization and tumoral volume. No toxicity was histologically observed.CONCLUSION:
The 3-microm PLGA microparticles provided significant enhancement in vitro and in vivo without any toxicity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Poliglicólico
/
Polímeros
/
Ultrassonografia Doppler
/
Ácido Láctico
/
Melanoma
/
Microesferas
Limite:
Animals
Idioma:
En
Revista:
Invest Radiol
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
França