[Influence of the mutants of hepatitis B surface antigen on the cell immunity].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 19(2): 124-7, 2005 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-16027776
ABSTRACT
OBJECTIVE:
To study the influence of the mutants of hepatitis B surface antigen on the cell immunity.METHODS:
The recombinant plasmids of NS2Swt, NS2S126, NS2S133, NS2S141 and NS2S145 were transfected into Chinese hamster ovary (CHO) cells and the expressed proteins were detected by means of ELISA. Following PHA-activated lymphoblasts proliferation assay and IFN-gamma, IL-2, IL-10 induction assay were done with these proteins.RESULTS:
It was identified that these proteins of HBsAg could stimulate human lymphoblasts proliferation. Besides, there were no significant difference between the mutants and the wild. It was deserved to point out that the HBsAg with T126S mutation could increase the expression of IFN-gamma in the culture medium while the HBsAg with M133T mutation induced more expression of IL-10.CONCLUSION:
The results suggested that the cellular immune response to mutants of HBV might not be strengthened or weakened. But it should not be ignored that HBV T126S or M133T mutation may assert a potential impact on the cell immunity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
/
Linfócitos
/
Proteínas Mutantes
/
Antígenos de Superfície da Hepatite B
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
Zh
Revista:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Assunto da revista:
VIROLOGIA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
China