Determination of the persistence of tetracycline antibiotics and their degradates in manure-amended soil using enzyme-linked immunosorbent assay and liquid chromatography-mass spectrometry.
J Agric Food Chem
; 53(18): 7165-71, 2005 Sep 07.
Article
em En
| MEDLINE
| ID: mdl-16131125
ABSTRACT
The persistence of manure-borne oxytetracycline in soil was investigated under field conditions. Soil cores were collected approximately once a month for over a period of two years and subsampled at depth increments of 0-5, 5-10, 10-15, 15-36, and 36-71 cm. Soil samples were analyzed by enzyme-linked immunosorbent assay (ELISA) and/or by liquid chromatography-mass spectrometry (LC-MS). Whereas LC-MS showed that oxytetracycline declined to <50% of its initial soil concentration after 3 weeks, ELISA showed that the total tetracyclines did not decline significantly 5 months after manure application. The differences between ELISA and LC-MS results are attributed to the broad cross-reactivity of the antibodies employed, which detect many structurally related tetracyclines, including their isomers and degradation products. Only trace amounts (< or = 1.0 microg/kg) of oxytetracycline were observed in the subsurface soil, and none was detected in water samples from field lysimeters, suggesting that oxytetracycline has low mobility in soil.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Solo
/
Tetraciclinas
/
Esterco
/
Antibacterianos
Idioma:
En
Revista:
J Agric Food Chem
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos