Expression of the cercosporin transporter, CFP, in tobacco reduces frog-eye lesion size.
Biotechnol Lett
; 27(20): 1543-50, 2005 Oct.
Article
em En
| MEDLINE
| ID: mdl-16245172
ABSTRACT
The cercosporin Major Facilitator Superfamily (MFS) transporter, CFP, under the control of the CaMV 35S promoter, was introduced into the Xanthi cultivar of tobacco by Agrobacterium-mediated transformation. CFP(+) transgenic plants were physically indistinguishable from non-transgenic Xanthi and progressed normally through growth to seed set. Accumulation of CFP in the leaf membrane fraction of CFP(+ )transgenic plants was associated with decreased cercosporin phytotoxicity. Frog-eye leaf lesions on CFP(+ )transgenic plants infected with Cercospora nicotianae conidia were smaller but were similar in number to those on non-transgenic plants. We conclude that transgenic expression of CFP may have relevance for a disease control strategy in Cercospora-plant pathosystems where cercosporin is implicated in pathogen virulence.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Membrana Transportadoras
/
Doenças das Plantas
/
Ascomicetos
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Nicotiana
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Proteínas Fúngicas
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Plantas Geneticamente Modificadas
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos