House dust mite-specific T cells in healthy non-atopic children.
Clin Exp Allergy
; 35(12): 1535-41, 2005 Dec.
Article
em En
| MEDLINE
| ID: mdl-16393318
ABSTRACT
BACKGROUND:
T-helper type 2 (Th2) cells play an important role in the pathogenesis of allergic diseases. Recent studies have demonstrated that allergen-specific T cells can also be found in the blood of healthy individuals. Both IL-10 and IFN-gamma might modulate the induction and maintenance of allergen-specific tolerance.AIM:
To study the phenotype and functional characteristics of allergen-specific T cells in healthy non-atopic children.METHODS:
Peripheral blood mononuclear cells (PBMC) from 13 symptomatic house dust mite (HDM)-allergic children and from nine matched healthy control children were stimulated with recombinant (r)Der p 2, a major allergen from HDMs.RESULTS:
Stimulation with rDer p 2 resulted in Th2 cytokine production in cultures of PBMC from allergic but not from healthy children. In contrast, IL-10 and IFN-gamma were induced in PBMC cultures from both healthy and HDM-allergic children. Intracellular staining revealed that IL-10 and IFN-gamma are largely produced by the same T cells. Stimulation of T cells from healthy children with rDer p 2 also induced expression of inducible costimulator (ICOS) on a small T cell subset.CONCLUSION:
Allergen-specific memory T cells from healthy non-atopic children produce IL-10 and IFN-gamma (but not Th2 cytokines) and express ICOS upon stimulation. These cells might be responsible for a normal immune balance after allergen encounter in non-atopics.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos T
/
Citocinas
/
Pyroglyphidae
/
Antígenos de Dermatophagoides
/
Hipersensibilidade
Tipo de estudo:
Observational_studies
Limite:
Child
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Female
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Humans
/
Male
Idioma:
En
Revista:
Clin Exp Allergy
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Bélgica