Development of mammalian embryos exposed to mixed-size nanoparticles.
Clin Exp Obstet Gynecol
; 32(4): 222-4, 2005.
Article
em En
| MEDLINE
| ID: mdl-16440818
ABSTRACT
Inhaled or ingested ultrafine nanoparticles and their effects on early pregnancy remain polemic. The objectives of the study were (a) to determine the embryotoxic effects of nanoparticles at the 2-cell stage and (b) to localize the internalized nanoparticles in the blastocyst. Thawed mouse 2-cell embryos (no. = 128) were exposed to either mixed-size polystyrene-based nanoparticles (11 million/ml) or control G1.3 medium and assessed after 72 hours. Additionally, blastocysts (no. = 146) were exposed to nanoparticles and analyzed. The results showed that the nanoparticles did not inhibit 2-cell embryo development to the blastocyst stage (89.4 vs 96.8%; treated vs control). There were no differences in hatching (34.8 vs 43.5%), implantation (13.6 vs 24.2%) and degeneration (10.6 vs 3.2%). Delayed exposure to nanoparticles showed similar percent hatching (40.7 vs 47.3%) and implantation (17.6 vs 20.0%). Although nanoparticles were internalized, embryo development was not inhibited suggesting a lack of embryotoxicity. During hatching, the larger nanoparticles adhered to the extruding blastocyst, preferentially on trophoblasts, but interference was insignificant. Exposure to polystyrene-based nanoparticles at the concentration tested are not associated with embryonic loss.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Blastocisto
/
Desenvolvimento Embrionário
/
Nanoestruturas
Limite:
Animals
Idioma:
En
Revista:
Clin Exp Obstet Gynecol
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos