Peroxisomal trans-2-enoyl-CoA reductase is involved in phytol degradation.
FEBS Lett
; 580(8): 2092-6, 2006 Apr 03.
Article
em En
| MEDLINE
| ID: mdl-16546181
ABSTRACT
Phytol is a naturally occurring precursor of phytanic acid. The last step in the conversion of phytol to phytanoyl-CoA is the reduction of phytenoyl-CoA mediated by an, as yet, unidentified enzyme. A candidate for this reaction is a previously described peroxisomal trans-2-enoyl-CoA reductase (TER). To investigate this, human TER was expressed in E. coli as an MBP-fusion protein. The purified recombinant protein was shown to have high reductase activity towards trans-phytenoyl-CoA, but not towards the peroxisomal beta-oxidation intermediates C241-CoA and pristenoyl-CoA. In conclusion, our results show that human TER is responsible for the reduction of phytenoyl-CoA to phytanoyl-CoA in peroxisomes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fitol
/
Peroxissomos
/
NADH NADPH Oxirredutases
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Holanda