Phenylethynylpyrene-labeled oligonucleotide probes for excimer fluorescence SNP analysis of 23S rRNA gene in clarithromycin-resistant Helicobacter pylori strains.
Mutat Res
; 599(1-2): 144-51, 2006 Jul 25.
Article
em En
| MEDLINE
| ID: mdl-16597449
ABSTRACT
The use of phenylethynylpyrene excimer forming pair in the design of specific fluorescent probes for determination of A2144G (A2143G and/or A2143C) mutations in 23S rRNA gene of Helicobacter pylori is described. Analysis of fluorescence spectra of model duplexes revealed optimal positions of fluorophore residues in the probe sequences for maximum efficiency of SNP detection. Application of excimer forming probes for analysis of DNA samples isolated from natural bacterial strains of H. pylori was demonstrated.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Bacteriano
/
RNA Ribossômico 23S
/
Helicobacter pylori
/
Polimorfismo de Nucleotídeo Único
Idioma:
En
Revista:
Mutat Res
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Federação Russa