Isocratic separation of ginsenosides by high-performance liquid chromatography on a diol column at subambient temperatures.
Anal Bioanal Chem
; 385(1): 96-104, 2006 May.
Article
em En
| MEDLINE
| ID: mdl-16598458
ABSTRACT
An improved high-performance liquid chromatographic method for separation of a number of ginsenosides has been developed. The influence of temperature (from 0 to 25 degrees C) on the retention and separation of the ginsenosides was studied by applying a binary mobile phase (acetonitrile/water, 8218 v/v) and a diol column (LiChrospher 100 Diol). The column temperature is one of the more important parameters for the retention and separation of the components investigated. Selected thermodynamic parameters, including changes of enthalpy (deltaH degrees) and entropy (deltaS degrees), were estimated from linear van't Hoff plots, and possible retention mechanisms were discussed. Moreover, the best separation conditions were selected based on optimization criteria including maximum retention time (t(R max)), minimum resolution (R(s min)), and relative resolution product (r). Temperature regions close to 14 degrees C offered the highest selectivity and almost equal distribution of the ginsenosides peaks across the chromatogram. Under such isocratic conditions, excellent separation of chromatographic standards and selected ginseng samples was achieved in less than 16 min.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Temperatura
/
Cromatografia Líquida de Alta Pressão
/
Ginsenosídeos
Idioma:
En
Revista:
Anal Bioanal Chem
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Japão