[Study on molecular genetic structure of Ael blood subgroup].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
; 23(2): 173-6, 2006 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-16604489
ABSTRACT
OBJECTIVE:
To study the ABO allele molecular characteristics of Ael blood subgroup.METHODS:
Five individuals of diagnosed as Ael blood subgroup were subjected to PCR amplify ABO alleles using four pairs of sequence-specific primers. Exon 6 and exon 7 at ABO locus of all samples were sequenced. An individual with AelB phenotype was chosen for further analysis of transcript structure of ABO gene.RESULTS:
Sequence analysis indicated one Ael phenotype sample with reported Ael01 allele, one Ael phenotype sample with an Ael05 allele, and two AelB and one Ael individuals did not contain referred A allele, but contain O01 or O02 allele with 261G deletion.CONCLUSION:
Molecular bases for the Ael have highly polymorphism. The mechanism responsible for the express weak A antigen of O allele with 261G deletion awaits to be elucidated.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polimorfismo Genético
/
Sistema ABO de Grupos Sanguíneos
/
DNA
/
Alelos
Limite:
Female
/
Humans
/
Male
Idioma:
Zh
Revista:
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
Assunto da revista:
GENETICA MEDICA
Ano de publicação:
2006
Tipo de documento:
Article