Role of gangliosides in the association of ErbB2 with lipid rafts in mammary epithelial HC11 cells.
FEBS J
; 273(8): 1821-30, 2006 Apr.
Article
em En
| MEDLINE
| ID: mdl-16623716
ABSTRACT
We analyzed the role of gangliosides in the association of the ErbB2 receptor tyrosine-kinase (RTK) with lipid rafts in mammary epithelial HC11 cells. Scanning confocal microscopy experiments revealed a strict ErbB2-GM3 colocalization in wild-type cells. In addition, analysis of membrane fractions obtained using a linear sucrose gradient showed that ErbB2, epidermal growth factor receptor (EGFR) and Shc-p66 (proteins correlated with the ErbB2 signal transduction pathway) were preferentially enriched in lipid rafts together with gangliosides. Blocking of endogenous ganglioside synthesis by (+/-)-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol hydrochloride ([D]-PDMP) induced a drastic cell-surface redistribution of ErbB2, EGFR and Shc-p66, within the Triton-soluble fractions, as revealed by linear sucrose-gradient analysis. This redistribution was partially reverted when exogenous GM3 was added to ganglioside-depleted HC11 cells. The results point out the key role of ganglioside GM3 in retaining ErbB2 and signal-transduction-correlated proteins in lipid rafts.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Receptor ErbB-2
/
Microdomínios da Membrana
/
Células Epiteliais
/
Gangliosídeo G(M3)
/
Glândulas Mamárias Animais
Tipo de estudo:
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
FEBS J
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Itália