Pancreatic cancer-derived S-100A8 N-terminal peptide: a diabetes cause?
Clin Chim Acta
; 372(1-2): 120-8, 2006 Oct.
Article
em En
| MEDLINE
| ID: mdl-16678810
ABSTRACT
BACKGROUND:
Our aim was to identify the pancreatic cancer diabetogenic peptide.METHODS:
Pancreatic tumor samples from patients with (n=15) or without (n=7) diabetes were compared with 6 non-neoplastic pancreas samples using SDS-PAGE.RESULTS:
A band measuring approximately 1500 Da was detected in tumors from diabetics, but not in neoplastic samples from non-diabetics or samples from non-neoplastic subjects. Sequence analysis revealed a 14 amino acid peptide (1589.88 Da), corresponding to the N-terminal of the S100A8. At 50 nmol/L and 2 mmol/L, this peptide significantly reduced glucose consumption and lactate production by cultured C(2)C(12) myoblasts. The 14 amino acid peptide caused a lack of myotubular differentiation, the presence of polynucleated cells and caspase-3 activation.CONCLUSIONS:
The 14 amino acid peptide from S100A8 impairs the catabolism of glucose by myoblasts in vitro and may cause hyperglycemia in vivo. Its identification in biological fluids might be helpful in diagnosing pancreatic cancer in patients with recent onset diabetes mellitus.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Pancreáticas
/
Proteínas S100
/
Diabetes Mellitus
Limite:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Clin Chim Acta
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Itália