A study of the genomic diversity of Plasmodium falciparum in Senegal. 1. Typing by Southern blot analysis.
Acta Trop
; 49(4): 281-92, 1991 Sep.
Article
em En
| MEDLINE
| ID: mdl-1684262
ABSTRACT
The genomic polymorphism of Plasmodium falciparum was investigated in a series of samples collected in Senegal during one transmission season. Restriction site polymorphism was studied by Southern blot analysis using six different probes. The patterns of the ribosomal RNA genes and of the gene coding for antigen 2L indicated a limited genomic polymorphism. Sequences hybridizing to the repeats of the Palo Alto/Wellcome serotype of S-antigen were found in one out of twelve isolates examined. This strain was shown to express the Palo Alto serotype. Restriction fragment length polymorphism was observed for the 332 gene and the 11.1 locus. The hybridization patterns showed that each sample had a distinct 11.1 locus. A comparison of three probes (332, 11.1 and rep20) detecting fragment length polymorphism indicated that maximum sensitivity was obtained using the subtelomeric repeats rep20; less sensitive patterns were observed using the 11.1 27 bp repeat probe. By using these three probes it was found that all samples were genetically distinct.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmodium falciparum
/
Variação Genética
/
Polimorfismo de Fragmento de Restrição
/
DNA de Protozoário
/
Malária Falciparum
Limite:
Animals
/
Humans
País/Região como assunto:
Africa
Idioma:
En
Revista:
Acta Trop
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
França