Quantitation of virus-induced (MLr) and normal (Thy.1.2) cell surface antigens in isolated plasma membranes and the extracellular ascites fluid of mouse leukemia cells.
Cancer Res
; 35(10): 2743-51, 1975 Oct.
Article
em En
| MEDLINE
| ID: mdl-168966
ABSTRACT
Plasma membranes were isolated by two methods from mouse leukemia cells containing mammary tumor virus-induced (MLr) and normal (Thy.1.2) antigens on their surfaces. A number of chemical components, enzymic activities, and the antigenic contents were determined in subcellular fractions and found to be specifically concentrated in the plasma membrane fractions. The major part of the cellular MLr, in contrast to Thy.1.2, was present in the 105,000 X gmax supernatant of the cell homogenate. This and other results indicate an easy release of the antigen from the plasma membrane. A considerable amount of MLr was also present in the ascites fluid, partly free and partly bound, supposedly in an immune complex that allowed the isolation of three components of similar molecular weights as mammary tumor virus components. The extracellular presence of MLr may illustrate that, by shedding of antigen, the tumor may protect itself against the immunological defense of the host.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucemia Experimental
/
Membrana Celular
/
Vírus do Tumor Mamário do Camundongo
/
Espaço Extracelular
/
Antígenos de Neoplasias
/
Antígenos Virais
Limite:
Animals
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1975
Tipo de documento:
Article