[Genotyping of the Plasmodium falciparum msp1 (block 2) and dhfr (codon 108) genes in field samples collected in four endemic Colombian localities]. / Genotipificación de los genes msp1 (bloque 2) y dhfr (codón108) de Plasmodium falciparum en muestras de campo recolectadas en cuatro localidades endémicas de Colombia.
Biomedica
; 26(1): 101-12, 2006 Mar.
Article
em Es
| MEDLINE
| ID: mdl-16929908
ABSTRACT
INTRODUCTION:
Plasmodium falciparum is a highly polymorphic parasite, which allows it to evade the host's immune response, spread drug resistance and favours transmission.OBJECTIVES:
To analyse the genetic diversity of P. falciparum populations in samples from four endemic localities in Colombia. MATERIALS ANDMETHODS:
123 blood samples were collected on filter paper from patients with non-complicated P. falciparum malaria during 2002 to 2004. The samples were genotyped using polymerase chain reaction with specific primers for the polymorphic region of block 2 of the msp1 gene and the 108 codon of the dhfr gene.RESULTS:
In msp1 block 2, 95.9% (118/123; 95% CI 90.8-98.7) of the samples harboured MAD20; 6.5% K1 (8/123; 95% CI 2.8-12.4) and 2.4% RO33 (3/123; 95% CI 0.5-6.9). For the dhfrgene the mutant allele N 108 was found in all the samples amplified, T 108 in 3.2% and the wild type S108 in 34.1%. Taking together all the results from both genes, 61.8% (76/123; 95% CI 52.6-70.4) of the samples were simple infections and 38.2% (47/123; 95% CI 29.6-47.4) were mixed infections. MAD20/N108-S108 (30.1%) was the most frequent combination among the latter.CONCLUSIONS:
Simple infections, i.e, a single allelic type in each one of the genes studied, prevailed among the circulating parasite populations. In this study the genetic composition of P. falciparum parasite populations was very homogeneous.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmodium falciparum
/
Tetra-Hidrofolato Desidrogenase
/
Proteína 1 de Superfície de Merozoito
Limite:
Animals
/
Humans
País/Região como assunto:
America do sul
/
Colombia
Idioma:
Es
Revista:
Biomedica
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Colômbia