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Validation of a competitive enzyme-linked immunosorbent assay for detection of antibodies against Babesia bovis.
Goff, Will L; Molloy, John B; Johnson, Wendell C; Suarez, Carlos E; Pino, Ignacio; Rhalem, Abdelkebir; Sahibi, Hamid; Ceci, Luigi; Carelli, Grazia; Adams, D Scott; McGuire, Travis C; Knowles, Donald P; McElwain, Terry F.
Afiliação
  • Goff WL; Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-6630, USA. wgoff@vetmed.wsu.edu
Clin Vaccine Immunol ; 13(11): 1212-6, 2006 Nov.
Article em En | MEDLINE | ID: mdl-16957062
A previously developed competitive enzyme-linked immunosorbent assay (cELISA) based on a species-specific, broadly conserved, and tandemly repeated B-cell epitope within the C terminus of rhoptry-associated protein 1 of Babesia bovis was refined and validated for use internationally. Receiver operating characteristic analysis revealed an assay with a specificity and positive predictive value of 100% and a sensitivity of 91.1%, with various negative predictive values depending on the level of disease prevalence. The cELISA was distributed to four different laboratories, along with a reference set of 100 defined bovine sera, including known-positive, known-negative, and field samples. Pairwise concordances among the four laboratories ranged from 94% to 88%. Analysis of variance of the resulting optical densities and a test of homogeneity indicated no significant difference among the laboratories. Overall, the cELISA appears to have the attributes necessary for international application.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Babesiose / Babesia bovis Tipo de estudo: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Clin Vaccine Immunol Assunto da revista: ALERGIA E IMUNOLOGIA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Babesiose / Babesia bovis Tipo de estudo: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Limite: Animals Idioma: En Revista: Clin Vaccine Immunol Assunto da revista: ALERGIA E IMUNOLOGIA / TECNICAS E PROCEDIMENTOS DE LABORATORIO Ano de publicação: 2006 Tipo de documento: Article País de afiliação: Estados Unidos