Chemisorption of proteins and their thiol derivatives onto gold surfaces: characterization based on electrochemical nonlinearity.
Biophys Chem
; 62(1-3): 63-72, 1996 Nov 29.
Article
em En
| MEDLINE
| ID: mdl-17029805
ABSTRACT
This study was conducted to monitor the electrochemical responses of two proteins (bovine serum albumin (BSA) and gelatin) and their thiol derivatives adsorbed onto gold (Au) electrodes, which were analyzed by a "nonlinear" impedance method. A sinusoidal voltage is applied to a protein-containing aqueous solution and the waveform of the output current is analyzed by fast Fourier transformation (FFT). The intensities of the higher harmonics in the FFT varied with the species of protein and their thiol derivatives, and with time. From the higher harmonics, voltage-dependent capacitance and conductance were quantitatively evaluated to differentiate the state of adsorbed protein. Adsorption and desorption characteristics of BSA and its thiol derivative on the Au surface were continuously measured by a quartz crystal microbalance (QCM) in situ. The microscopic state of thiol-derivatized BSA adsorbed onto the Au surface was imaged by atomic force microscopy (AFM). In general, thiol-derivatized proteins were tightly adsorbed on the Au surface and showed no desorption. The present electrochemical measurements clearly differentiated adsorption characteristics of physically adsorbed (physisorbed) and chemically adsorbed (chemisorbed) proteins on Au surfaces.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Biophys Chem
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Japão