Electrophoretic determination of albumin in urine using on-line concentration techniques.
J Chromatogr A
; 1150(1-2): 332-8, 2007 May 25.
Article
em En
| MEDLINE
| ID: mdl-17126842
ABSTRACT
To improve the sensitivity of the UV-detection for the determination of trace amounts of albumin by capillary zone electrophoresis (CZE), five on-line preconcentration techniques, including field-amplified sample stacking (FASS), head-column field-amplified sample stacking (HC-FASS), stacking with a polymer solution, dynamic pH junction and large volume sample stacking (LVSS) with reversed polarity, were compared. Sensitivity enhancement factor and reproducibility were two factors that were used to assess the suitability of each method. To minimize protein adsorption on the capillary wall, capillaries were covalently modified with anionic polymer, poly(sulfopropylmethacrylate) coating. All used methods have good reproducibility. The maximum sensitivity enhancement factor (about 67-fold in terms of peak heights) was achieved with LVSS technique. The concentration limit of detection (LOD) (S/N=3) for the human serum albumin obtained with the optimized LVSS approach was 15 microg/ml with UV-detection. The method was further evaluated for the analysis of urine samples with gel-filtration-based sample-desalting procedure.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Eletroforese Capilar
/
Albuminúria
Limite:
Humans
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Federação Russa