The Sp family of transcription factors regulates the human laminin alpha1 gene in JAR choriocarcinoma cells.
Biochim Biophys Acta
; 1759(11-12): 573-9, 2006.
Article
em En
| MEDLINE
| ID: mdl-17141338
ABSTRACT
Laminin-111 (alpha1beta1gamma1) is the major component of the embryonic and extra-embryonic basement membrane. The laminin alpha1 chain shows a restricted and developmentally regulated expression in basement membranes of distinct epithelial tissues while beta1 and gamma1 chains have a wide tissue distribution. To understand how human laminin alpha1 chain expression is controlled, we cloned and characterized the 5'-flanking region of the human laminin alpha1 (LAMA1) gene. Transfection studies using serially deleted promoter constructs and JAR choriocarcinoma cells revealed that the minimal promoter fragment resided in the +31 to -206 region, which contains a number of GC- and GT/A-rich motifs for the binding of the Sp family of transcription factors. Electrophoretic mobility shift assays and mutational analyses revealed that Sp1 and Sp3 bound specifically to these elements and are important for the promoter activity. Furthermore, we showed that Krüppel-like factors KLF4 and KLF6 also activate transcription of the human LAMA1 gene. Chromatin immunoprecipitation analysis demonstrated recruitment of these transcription factors to the promoter region. These results indicate that transcription of the human LAMA1 gene is controlled by a combination of the actions of Sp1/Sp3 and Krüppel-like factors, KLF4 and KLF6.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Regulação Neoplásica da Expressão Gênica
/
Fator de Transcrição Sp1
/
Laminina
/
Fator de Transcrição Sp3
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Japão