Increase of fluorescence anisotropy upon self-assembly in headgroup-labeled surfactants.
Macromol Biosci
; 7(2): 189-94, 2007 Feb 12.
Article
em En
| MEDLINE
| ID: mdl-17295406
ABSTRACT
The change in fluorescence anisotropy upon micellization in headgroup-labeled surfactants is investigated. After eliminating the likelihood of depolarizing RET, anisotropy is shown to increase upon self-assembly due to increased rotational correlation times of the fluorophore. This is shown using two surfactant-fluorophore systems. Anisotropy in NBD-labeled phospholipids is studied both in chloroform (unaggregated) and in water (unilamellar vesicles), while in tryptophan-containing peptide-amphiphiles, the variation of anisotropy with concentration leads to a reasonable measurement of CAC. Anisotropy increase is shown to be largely the product of increased rotational correlation times for the fluorophore, relative to its tau. These results serve as a basis for future work that measures the amount of depolarizing energy transfer, characterizing distances between similar fluorescent headgroups on mixed micelles.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Tensoativos
/
Polarização de Fluorescência
/
Micelas
Idioma:
En
Revista:
Macromol Biosci
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos