Development and integration of an immunoaffinity monolithic disk for the on-line solid-phase extraction and HPLC determination with fluorescence detection of aflatoxin B1 in aqueous solutions.
J Pharm Biomed Anal
; 44(2): 396-403, 2007 Jun 28.
Article
em En
| MEDLINE
| ID: mdl-17317069
ABSTRACT
The development and characterization of an anti-aflatoxin B1 (anti-AFB1) immunoaffinity monolithic disk is reported. Polyclonal anti-AFB1 was covalently immobilized in batch on an epoxy-activated monolithic Convective Interaction Media (CIM) disk (12 mm x 3 mm i.d.) by a one-step reaction via epoxy groups of the polymer surface. 0.96 mg of antibody were immobilized and the binding capacity of the CIM disk was determined by frontal analysis. The CIM disk was coupled through a switching valve to a reversed-phase column, namely Chromolith Performance RP-18e. A fully automated HPLC method with fluorescence detection for the determination of aflatoxin B1 in aqueous solution was developed. The total analysis time with the integrated system is 46 min and the retention time of AFB1 is approximately 29 min. The binding capacity of the immunoaffinity disk was evaluated in terms of linearity, precision and accuracy of the extraction procedure. The immunoaffinity support was stable after repeated runs.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoquímica
/
Aflatoxina B1
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Itália