B-RAF mutations are a rare event in pituitary adenomas.
J Endocrinol Invest
; 30(1): RC1-3, 2007 Jan.
Article
em En
| MEDLINE
| ID: mdl-17318013
ABSTRACT
Pituitary tumors are a relatively common neoplasia whose pathogenesis is still largely unknown. Recent studies have revealed frequent activating mutations of the gene for B-RAF, an effector of Ras protein in the mitogen-activated protein kinase pathway, in several malignancies, including melanoma, thyroid, colorectal and ovarian cancer. However, analyses of B-RAF mutations in pituitary tumors have not been reported so far. Therefore, in the present study we have investigated the presence of the B-RAF mutations, by polymerase chain reaction (PCR) amplification of the hot spot exons 11 and 15, followed by direct sequencing, in 50 human pituitary adenomas, including 25 NFPA and 25 secreting adenomas (10 GH, 5 PRL, 6 LH and/or FSH, 4 GH/PRL). We found only one V600E mutation in a NFPA sample, suggesting that B-RAF mutations are a rare event in pituitary tumorigenesis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Hipofisárias
/
Adenoma
/
Proteínas Proto-Oncogênicas B-raf
/
Mutação
Limite:
Humans
Idioma:
En
Revista:
J Endocrinol Invest
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Itália