Fluorescent stain method for the simultaneous determination of mitochondrial potential and integrity of plasma and acrosomal membranes in boar sperm.
Reprod Domest Anim
; 42(2): 190-4, 2007 Apr.
Article
em En
| MEDLINE
| ID: mdl-17348977
ABSTRACT
The purpose of this study was to validate a technique for simultaneous evaluation of the plasma, acrosomal and mitochondrial membranes in boar spermatozoa, using an association of fluorescent probes Propidium iodide (PI), fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA) and JC-1. Three ejaculates from each of four different boars, all showing motility >or=80% and abnormal morphology
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Motilidade dos Espermatozoides
/
Espermatozoides
/
Suínos
/
Acrossomo
/
Mitocôndrias
Limite:
Animals
Idioma:
En
Revista:
Reprod Domest Anim
Assunto da revista:
MEDICINA REPRODUTIVA
/
MEDICINA VETERINARIA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Brasil