New triphosphate conjugates bearing reporter groups: labeling of DNA fragments for microarray analysis.
Bioconjug Chem
; 18(3): 886-93, 2007.
Article
em En
| MEDLINE
| ID: mdl-17352454
A simple and convenient method for incorporation of fluorescent or ligand groups into 3'-termini of DNA fragments is proposed. A set of triphosphoric acid monoesters bearing fluorescent groups or biotin attached to the triphosphate fragment through linkers of different lengths and structures was synthesized. All the compounds were substrates for calf thymus terminal deoxynucleotidyltransferase and were used for incorporation of marker groups into 3'-termini of DNA fragments. The compounds were successfully applied for DNA labeling during post-PCR target preparation for microarray analysis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polifosfatos
/
Sondas de DNA
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Corantes Fluorescentes
Limite:
Animals
Idioma:
En
Revista:
Bioconjug Chem
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2007
Tipo de documento:
Article