Conformation of DNA-DNA polymerase I complex observed by scanning tunneling microscopy.
J Biomol Struct Dyn
; 9(2): 233-8, 1991 Oct.
Article
em En
| MEDLINE
| ID: mdl-1741960
ABSTRACT
The conformation of a complex of a 41 mer/31 mer DNA fragment and the Klenow fragment of DNA polymerase I of Escherichia coli was studied by scanning tunnelling microscopy (STM). The results shows that near two turns of double helix of this DNA fragment was outside of enzyme while another part containing more than one turn of helix and 10 nucleotides single strand was combined with enzyme. The dimension and shape of DNA polymerase I (KF) in complex were different from that of free enzyme. The conformation of DNA-DNA polymerase I (KF) complex and the application of STM in studying structure of complex of DNA polymerase with DNA were discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Bacteriano
/
DNA Polimerase I
Idioma:
En
Revista:
J Biomol Struct Dyn
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
China