An experimentally derived database of candidate Ras-interacting proteins.
J Proteome Res
; 6(5): 1806-11, 2007 May.
Article
em En
| MEDLINE
| ID: mdl-17439166
ABSTRACT
We used a TAP-tag approach to identify candidate binding proteins for the related Ras family GTPases H-Ras, R-Ras, and Rap1A. Protein complexes were isolated from mouse fibroblasts, and component proteins were identified by a combination of nanoflow HPLC and tandem mass spectrometry. H-Ras was found to associate with numerous cytoskeletal proteins including talin-1. R-Ras and Rap1A each associated with various signaling molecules, many of which are membrane-associated. Thus, we have established the first database of potential Ras interactors in mammalian cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia de Afinidade
/
Proteínas ras
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Proteínas rap1 de Ligação ao GTP
/
Bases de Dados de Proteínas
Limite:
Animals
Idioma:
En
Revista:
J Proteome Res
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos