[Roles of CD8+ CD28- T regulatory cells in acute infectious mononucleosis in children].
Zhonghua Er Ke Za Zhi
; 45(3): 208-11, 2007 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-17504627
ABSTRACT
OBJECTIVE:
To explore the role of CD(8)(+)CD(28)(-) T regulatory cells (Tr) in the immunological pathogenesis of acute infection with Epstein-Barr virus in children.METHODS:
The present study enrolled 25 children with infectious mononucleosis (IM) and 25 age-matched healthy children. Flow cytometric analysis was performed to detect the percentage of CD(3)(+), CD(3)(+)CD(4)(+), CD(3)(+)CD(8)(+), CD(8)(+)CD(28)(+) by determining the ratio of positive cells in lymphocytes. Reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR were used to analyze IL-6, IL-10, IFN-gamma expression in CD(8)(+)CD(28)(-) Tr cells and ILT-3, ILT-4 expression in monocytes/macrophages.RESULTS:
The proportions of CD(8)(+)CD(28)(-)T cells in children with acute-phase IM was significantly higher than those in the controls (P < 0.01). The expression level of IL-6, IL-10, IFN-gamma, ILT-3, ILT-4 mRNA significantly increased compared to those of the controls (P < 0.01).CONCLUSION:
The CD(28) expressed on CD(8)(+) T cells in vivo is gradually lost with age and CD(8)(+)CD(28)(-) cells increase up 50% to adult. EBV can directly infect B cells, trigger CD(8)(+) CTL response and destroy the target cells to cause serious immunopathological lesion. Therefore we speculate that the expansion of CD(8)(+)CD(28)(-) Tr cells in children with IM may be an adaptive immune response to avoid serious inflammation and autoimmune reactions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfócitos T Reguladores
/
Antígenos CD28
/
Linfócitos T CD8-Positivos
/
Mononucleose Infecciosa
Tipo de estudo:
Observational_studies
Limite:
Child
/
Child, preschool
/
Female
/
Humans
/
Male
Idioma:
Zh
Revista:
Zhonghua Er Ke Za Zhi
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
China