Kinetic analysis of nanoparticulate polyelectrolyte complex interactions with endothelial cells.
Biomaterials
; 28(26): 3843-55, 2007 Sep.
Article
em En
| MEDLINE
| ID: mdl-17560645
ABSTRACT
A non-toxic, nanoparticulate polyelectrolyte complex (PEC) drug delivery system was formulated to maintain suitable physicochemical properties at physiological pH. Toxicity, binding, and internalization were evaluated in relevant microvascular endothelial cells. PEC were non-toxic, as indicated by cell proliferation studies and propidium iodide staining. Inhibitor studies revealed that PEC were bound, in part, via heparan sulfate proteoglycans and internalized through macropinocytosis. A novel, flow cytometric, Scatchard protocol was established and showed that PEC, in the absence of surface modification, bind cells non-specifically with positive cooperativity, as seen by graphical transformations.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sobrevivência Celular
/
Células Endoteliais
/
Eletrólitos
/
Nanopartículas
Limite:
Humans
Idioma:
En
Revista:
Biomaterials
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos