Heterogeneity of cathepsin B and stefin A expression in Gleason pattern 3+3 (score 6) prostate cancer needle biopsies.
Anticancer Res
; 27(3B): 1407-13, 2007.
Article
em En
| MEDLINE
| ID: mdl-17595755
ABSTRACT
BACKGROUND:
There is a significant positive association of increased ratios of cathepsin B to its endogenous inhibitor stefin (cystatin) A in prostatectomy tumors with pelvic lymph node metastases. Needle biopsy diagnosis of prostate cancer is critical in initial treatment selection. The objective was to characterize cathepsin B and stefin A immunostaining patterns in needle biopsies of histologically similar Gleason pattern 3+3 (score 6) foci in relation to pretreatment clinical data. MATERIALS ANDMETHODS:
Immunostaining of cathepsin B and stefin A of 65 biopsy sections were imaged, quantified and analyzed with Student's t-test (p < 0.05).RESULTS:
Patients had T1c to T3b clinical stages and pre-surgery total prostate-specific antigen serum levels from 1.25 to 20.0 ng/ml. Cathepsin B and stefin A reaction products were found in the cytoplasm of basal and columnar/cuboidal cells of benign prostatic hyperplasia (BPH), prostatic intraepithelial neoplasia (PIN) and neoplastic cells. Ratios of cathepsin B to stefin A were significantly higher in prostate cancer when compared to that in BPH and PIN glands.CONCLUSION:
Small foci of Gleason pattern 3+3 tumors in needle biopsies have heterogeneous cathepsin B and stefin A immunostaining. Stratification of these tumors in relation to clinical stage by cathepsin B and stefin A may assist in treatment selection.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Próstata
/
Catepsina B
/
Cistatinas
Limite:
Aged
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Anticancer Res
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos