[Spectral analysis and synthesis of artificial antigen of the organophosphors pesticide methamidophos].
Guang Pu Xue Yu Guang Pu Fen Xi
; 27(5): 881-5, 2007 May.
Article
em Zh
| MEDLINE
| ID: mdl-17655094
ABSTRACT
In the present paper, a hapten of methamidophos was synthesized and conjugated with KLH by active ester method, thus the first artificial antigen was obtained. By diazotization method methamidophos conjugated with BSA, and the second artificial antigen was obtained. The synthesized haptens were characterized by MS, IR and 1H NMR, and the two artificial antigens were determined by the method of IR spectrum. The result implied that both the artificial antigens have absorbance peaks of hapten and protein, indicating that they were prepared successfully. This could provide evidence that the method of IR spectrum can be used to determine whether the artificial antigens are synthesized successfully.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Compostos Organofosforados
/
Compostos Organotiofosforados
/
Praguicidas
/
Espectrofotometria Infravermelho
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Espectroscopia de Ressonância Magnética
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Espectrometria de Massas por Ionização por Electrospray
/
Antígenos
Limite:
Animals
Idioma:
Zh
Revista:
Guang Pu Xue Yu Guang Pu Fen Xi
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
China